Free shipping starts now, no minimum, no coupons required!

Anti-Human TRPV2 (VRL1) (extracellular) Antibody

Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B

Cat #: ACC-139
Alternative Name Vanilloid receptor-like protein 1, VRL-1, Osm-9-like TRP channel 2, OTRPC2, Strech-activated channel 2B, SAC2B
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h
Immunogen
  • Peptide (C)KQAAPHLKAEVGNS, corresponding to amino acid residues 417-430 of human TRPV2 (Accession Q9Y5S1). 1st extracellular loop.
Accession (Uniprot) Number Q9Y5S1
Gene ID 51393
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Human only.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Specificity The antibody will not recognize the protein from rat or mouse samples.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ifc, lci, wb
May also work in: ic*, ih*, ip*
Western blot
  • Western blot analysis of human HMC3 (lanes 1 and 5), Jurkat (lanes 2 and 6), HepG2 (lanes 3 and 7) and K562 (lanes 4 and 8) cell lysates:
    Western blot analysis of human HMC3 (lanes 1 and 5), Jurkat (lanes 2 and 6), HepG2 (lanes 3 and 7) and K562 (lanes 4 and 8) cell lysates:
    1-4. Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139), (1:200).
    5-8. Anti-Human TRPV2 (VRL1) (extracellular) Antibody, preincubated with Human TRPV2/VRL1 (extracellular) Blocking Peptide (#BLP-CC139).
Indirect flow cytometry
  • Cell surface detection of TRPV2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    Cell surface detection of TRPV2 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + goat-anti-rabbit-FITC.
    ___ Cells + Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139), (2.5μg) + goat-anti-rabbit-FITC.
References
  1. Montell, C. et al. (2002) Mol. Cell 9, 229.
  2. Clapham, D.E. (2003) Nature 426, 517.
  3. Moran, M.M. et al. (2004) Curr. Opin. Neurobiol14, 362.
  4. Clapham, D.E. et al. (2003) Pharmacol. Rev55, 591.
  5. Gunthorpe, M.J. et al. (2002) Trends Pharmacol. Sci. 23, 183.
  6. Tominaga, M. and Caterina, M.J. et al. (2004) J. Neurobiol61, 3.
  7. Voets, T. et al. (2004) Nature 430, 748.
  8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.
  9. Muraki, K. et al. (2003) Circ. Res93, 829.
  10. Shimosato, G. et al. (2005) Pain 119, 225.
  11. Saunders, C.I. et al. (2007) Mol. Immunol44, 1429.
  12. Caterina, M.J. et al. (1999) Nature 398, 436.
Scientific background

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have six putative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-termini of all TRPs are intracellular3.

According to IUPHAR, the TRP family is comprised of numerous subfamilies on the basis of sequence homology; TRPC, TRPM, TRPV, TRPA, TRPML, and TRPP1-4. The TRPV subfamily consists of six members, TRPV1-65.

Four members of the TRPV family have been described as thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17°C) to noxious heat (>52°C) 6,7. Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52°C and is considered to play an essential role in the perception of high-intensity noxious heat stimulation8-10. TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway8. The TRPV2 channel is expressed in DRG neurons, different brain regions and non-neuronal tissues such as the spleen, lung and intestine and in components of the immune system5,11,12.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Anti-Human TRPV2 (VRL1) (extracellular) Antibody (#ACC-139) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize TRPV2 from human samples only. It will not recognize the protein from rat or mouse samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Shipping and Ordering information

Resources