Anti-KV1.3 (KCNA3) (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 3
    Cat #: APC-101
    Alternative Name Potassium voltage-gated channel subfamily A member 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    Immunogen
    • Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
    • Anti-KV1.3 (KCNA3) (extracellular) Antibody
    Accession (Uniprot) Number P22001
    Gene ID 3738
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 12/14 amino acid residues identical.
    RRID AB_2040149.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 5 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, ifc, ih, ip, lci, wb
    Western blot
    • Anti-KV1.3 (KCNA3) (extracellular) Antibody
      Western blot analysis of rat brain membranes:  
      1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  
      2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the negative control antigen.
    • Anti-KV1.3 (KCNA3) (extracellular) Antibody
      Western blot analysis of human Jurkat T cells:
      1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
      2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the negative control antigen.
    Immunoprecipitation
    • Human lung carcinoma cell line A549 isolated nuclei (Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.).
    Immunohistochemistry
    • Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
    Immunocytochemistry
    • Human B-cell lymphoma cells (Raji cells), (1:100) (Kawano, Tet al. (2009) Biol. Pharm. Bull. 32, 345.).
    Indirect flow cytometry
    • Anti-KV1.3 (KCNA3) (extracellular) Antibody
      Cell surface detection of KV1.3 in live intact human T cell leukemia (Jurkat) cell line:
      ___ Cells + goat-anti-rabbit-PE.
      ___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:25) + goat-anti-rabbit-PE.
    • Human T cells (Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17414.).
    • The negative control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    • Anti-KV1.3 (KCNA3) (extracellular) Antibody
      Expression of KV1.3 in HEK 293 transfected cells
      Cell surface detection of KV1.3 in live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50).
      Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
    References
    1. Chandy, K.G. et al. (2001) Toxicon 39, 1269.
    2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
    Scientific background

    KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.

    For research purposes only, not for human use

    Applications

    Specifications

    Scientific Background

    Citations

    Citations
    Western blot citations
    1. Human Jurkat cell lysate.
      Szabo, I. et al. (2015) Cell. Physiol. Biochem. 37, 965.
    2. Human lung carcinoma cell line A549 isolated nuclei.
      Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
    3. Human lymphomas (1:200).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    4. Human B-CLL cells.
      Leanza, L. et al. (2013) Leukemia 27, 1782.
    5. Human Jurkat T-cells (1:300).
      Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
    Immunoprecipitation citations
    1. Human lung carcinoma cell line A549 isolated nuclei.
      Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
    Immunohistochemistry citations
    1. Mouse cerebral cortex.
      Duque, A. et al. (2013) Develop. Neurobiol. 73, 841.
    2. Human lymphomas (1:70).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    3. Human acute T-lymphoblastic lymphomas (1:300).
      Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
    4. Mouse retina (1:200).
      Cangiano, L. et al. (2007) PLoS ONE 2, 1327.
    Immunocytochemistry citations
    1. HEK 293 transfected cells.
      Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569.
    2. Human umbilical vascular endothelial cells (HUVECs) (1:100).
      Yang, L. et al. (2013) Toxicol. Lett. 223, 16.
    3. Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes.
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    4. Human B-cell lymphoma cells (Raji cells), (1:100).
      Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.
    Indirect flow cytometry citations
    1. Human T cells.
      Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17414.
    More product citations
    1. Hajdu, P. et al. (2015) Mol. Biol. Cell 26, 1640.
    2. Gazula, V-R. et al. (2010) J. Comp.Neurol. 518, 3205.
    3. Vicente, R. et al. (2008) J. Biol. Chem. 283, 8756.
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