Anti-KV1.3 (KCNA3) (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 3
    Cat #: APC-101
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession number P22001). Extracellular loop between domains S1 and S2.
    Accession (Uniprot) Number P22001
    Gene ID 3738
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 5 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, ip, lci, wb
    Western blot
    Western blot analysis of rat brain membranes:  
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.
    Western blot analysis of human Jurkat T cells:
    1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
    2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.
    Immunoprecipitation
    Human lung carcinoma cell line A549 isolated nuclei (Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.).
    Immunohistochemistry
    Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
    Immunocytochemistry
    Human B-cell lymphoma cells (Raji cells), (1:100) (Kawano, Tet al. (2009) Biol. Pharm. Bull. 32, 345.).
    Indirect flow cytometry
    Indirect flow cytometry analysis of live intact human T cell leukemia (Jurkat) cell line:
    ___ Cells + goat-anti-rabbit-PE.
    ___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:25) + goat-anti-rabbit-PE.
    Human T cells (Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17414.).
    The control antigen is not suitable for this application.
    Live cell imaging / Immunocytochemistry
    Expression of KV1.3 in HEK 293 transfected cells
    Immunocytochemical staining of live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50).
    Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
    References
    1. Chandy, K.G. et al. (2001) Toxicon. 39, 1269.
    2. Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
    Scientific background

    KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.  

    Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs. The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.

    For research purposes only, not for human use
    Citations
    Western blot citations
    1. Human Jurkat cell lysate.
      Szabo, I. et al. (2015) Cell. Physiol. Biochem. 37, 965.
    2. Human lung carcinoma cell line A549 isolated nuclei.
      Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
    3. Human lymphomas (1:200).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    4. Human B-CLL cells.
      Leanza, L. et al. (2013) Leukemia 27, 1782.
    5. Human Jurkat T-cells (1:300).
      Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
    Immunoprecipitation citations
    1. Human lung carcinoma cell line A549 isolated nuclei.
      Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
    Immunohistochemistry citations
    1. Mouse cerebral cortex.
      Duque, A. et al. (2013) Develop. Neurobiol. 73, 841.
    2. Human lymphomas (1:70).
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    3. Human acute T-lymphoblastic lymphomas (1:300).
      Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
    4. Mouse retina (1:200).
      Cangiano, L. et al. (2007) PLoS ONE 2, 1327.
    Immunocytochemistry citations
    1. HEK 293 transfected cells.
      Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569.
    2. Human umbilical vascular endothelial cells (HUVECs) (1:100).
      Yang, L. et al. (2013) Toxicol. Lett. 223, 16.
    3. Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes.
      Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
    4. Human B-cell lymphoma cells (Raji cells), (1:100).
      Kawano, Tet al. (2009) Biol. Pharm. Bull. 32, 345.
    Indirect flow cytometry citations
    1. Human T cells.
      Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17414.
    More product citations
    1. Hajdu, P. et al. (2015) Mol. Biol. Cell 26, 1640.
    2. Gazula, V-Ret al. (2010) J. Comp.Neurol. 518, 3205.
    3. Vicente, Ret al. (2008) J. Biol. Chem. 283, 8756.
    Related Products
      • Products for control experiments
        1. Anti-KV1.3 (KCNA3) Antibody (#APC-002). This antibody recognizes a different epitope and thus can also be used as a control for detecting KV1.3.
      • Antibodies for immuno-colocalization experiments
        1. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-101.
        2. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B). A biotin labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.