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2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.

2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.

___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:25) + goat-anti-rabbit-PE.

Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
- Chandy, K.G. et al. (2001) Toxicon. 39, 1269.
- Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. It is in these cells (particularly T lymphocytes) that KV1.3 function has centered a lot of attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.
Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs. The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.
Application key:
Species reactivity key:
Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.
- Human Jurkat cell lysate.
Szabo, I. et al. (2015) Cell. Physiol. Biochem. 37, 965. - Human lung carcinoma cell line A549 isolated nuclei.
Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547. - Human lymphomas (1:200).
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human B-CLL cells.
Leanza, L. et al. (2013) Leukemia 27, 1782. - Human Jurkat T-cells (1:300).
Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
- Human lung carcinoma cell line A549 isolated nuclei.
Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
- Mouse cerebral cortex.
Duque, A. et al. (2013) Develop. Neurobiol. 73, 841. - Human lymphomas (1:70).
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human acute T-lymphoblastic lymphomas (1:300).
Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273. - Mouse retina (1:200).
Cangiano, L. et al. (2007) PLoS ONE 2, 1327.
- HEK 293 transfected cells.
Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569. - Human umbilical vascular endothelial cells (HUVECs) (1:100).
Yang, L. et al. (2013) Toxicol. Lett. 223, 16. - Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes.
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human B-cell lymphoma cells (Raji cells), (1:100).
Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.
- Human T cells.
Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17414.
- Hajdu, P. et al. (2015) Mol. Biol. Cell 26, 1640.
- Gazula, V-R. et al. (2010) J. Comp.Neurol. 518, 3205.
- Vicente, R. et al. (2008) J. Biol. Chem. 283, 8756.
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- Anti-KV1.3 (KCNA3) Antibody (#APC-002). This antibody recognizes a different epitope and thus can also be used as a control for detecting KV1.3.
Products for control experiments
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- Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005) is raised in guinea pig and can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-101.
- Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B). A biotin labeled primary antibody. It can be used in immuno-colocalization studies in conjunction with any of our antibodies raised in rabbit.
Antibodies for immuno-colocalization experiments
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- Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F). This FITC-conjugated antibody can be used to detect KV1.3 in live cell flow cytometry.
Antibodies for live cell flow cytometry experiments
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- Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#AGP-005) is raised in guinea pig and can be used in colocalization studies in conjunction with any of our antibodies raised in rabbit. This antibody has been raised against the same epitope as #APC-101.
- Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F). A fluorescent labeled primary antibody. It can be used to detect KV1.3 in live cells.
- Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B). A biotin labeled primary antibody. This antibody is the biotin-labeled version of #APC-101 and is ideal to detect KV1.3 in live cells.
Antibodies for live cell imaging experiments