Overview
Cat #:
APC-101
Alternative Name Potassium voltage-gated channel subfamily A member 3
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
Accession (Uniprot) Number P22001
Gene ID 3738
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat, mouse - 12/14 amino acid residues identical.
RRID AB_2040149.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.8 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ifc, ih, ip, lci, wb
Western blot
Western blot analysis of rat brain membranes:1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:500).
2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
Western blot analysis of human Jurkat T cells:1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
Immunoprecipitation
- Human lung carcinoma cell line A549 isolated nuclei (Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.).
Immunohistochemistry
- Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
Immunocytochemistry
- Human B-cell lymphoma cells (Raji cells), (1:100) (Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.).
Indirect flow cytometry
Cell surface detection of KV1.3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-APC.
___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (5μg) + goat-anti-rabbit-APC.- Human T cells (Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17414.).
- The blocking peptide is not suitable for this application.
Cell surface detection of KV1.3 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + goat-anti-rabbit-FITC.
___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (2.5μg) + goat-anti-rabbit-FITC.
Live cell imaging / Immunocytochemistry
Expression of KV1.3 in HEK 293 transfected cellsCell surface detection of KV1.3 in live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50).
Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
References
- Chandy, K.G. et al. (2001) Toxicon 39, 1269.
- Koo, G.C. et al. (1997) J. Immunol. 158, 5120.
Scientific background
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.
Lyophilized Powder
Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.
For research purposes only, not for human use
Last Update: 08/08/2023
Applications
Citations
Citations
Powered by BiozKO validation citations
- Immunocytochemistry of mouse bone marrow-derived neutrophils. Tested in Kv1.3 -/- mice.
Immler, R. et al. (2022) Cardiovasc. Res. 118, 1289.
Western blot citations
- Human Jurkat cell lysate.
Szabo, I. et al. (2015) Cell. Physiol. Biochem. 37, 965. - Human lung carcinoma cell line A549 isolated nuclei.
Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547. - Human lymphomas (1:200).
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human B-CLL cells.
Leanza, L. et al. (2013) Leukemia 27, 1782. - Human Jurkat T-cells (1:300).
Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
Immunoprecipitation citations
- Human lung carcinoma cell line A549 isolated nuclei.
Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
Immunohistochemistry citations
- Mouse cerebral cortex.
Duque, A. et al. (2013) Develop. Neurobiol. 73, 841. - Human lymphomas (1:70).
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human acute T-lymphoblastic lymphomas (1:300).
Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273. - Mouse retina (1:200).
Cangiano, L. et al. (2007) PLoS ONE 2, 1327.
Immunocytochemistry citations
- HEK 293 transfected cells.
Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569. - Human umbilical vascular endothelial cells (HUVECs) (1:100).
Yang, L. et al. (2013) Toxicol. Lett. 223, 16. - Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes.
Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779. - Human B-cell lymphoma cells (Raji cells), (1:100).
Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.
Indirect flow cytometry citations
- Human T cells.
Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17414.
More product citations
- Hajdu, P. et al. (2015) Mol. Biol. Cell 26, 1640.
- Gazula, V-R. et al. (2010) J. Comp.Neurol. 518, 3205.
- Vicente, R. et al. (2008) J. Biol. Chem. 283, 8756.
