Anti-KV1.3 (KCNA3) (extracellular) Antibody

Potassium voltage-gated channel subfamily A member 3
Cat #: APC-101
Alternative Name Potassium voltage-gated channel subfamily A member 3
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
    Accession (Uniprot) Number P22001
    Gene ID 3738
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse - 12/14 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 5 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ifc, ih, ip, lci, wb
      • Western blot analysis of rat brain membranes:  
        1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  
        2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.
        Western blot analysis of human Jurkat T cells:
        1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:200)
        2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with the control peptide antigen.
      • Human lung carcinoma cell line A549 isolated nuclei (Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.).
      • Mouse retina (1:200) (Cangiano, L. et al. (2007) PLoS ONE 2, 1327.).
      • Human B-cell lymphoma cells (Raji cells), (1:100) (Kawano, Tet al. (2009) Biol. Pharm. Bull. 32, 345.).
      • Indirect flow cytometry analysis of live intact human T cell leukemia (Jurkat) cell line:
        ___ Cells + goat-anti-rabbit-PE.
        ___ Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:25) + goat-anti-rabbit-PE.
        Human T cells (Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103, 17414.).
        The control antigen is not suitable for this application.
      • Expression of KV1.3 in HEK 293 transfected cells
        Cell surface detection of KV1.3 in live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50).
        Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
      • KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 09/04/2019

    Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.

    For research purposes only, not for human use
    Citations
      • Human Jurkat cell lysate.
        Szabo, I. et al. (2015) Cell. Physiol. Biochem. 37, 965.
      • Human lung carcinoma cell line A549 isolated nuclei.
        Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
      • Human lymphomas (1:200).
        Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
      • Human B-CLL cells.
        Leanza, L. et al. (2013) Leukemia 27, 1782.
      • Human Jurkat T-cells (1:300).
        Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
      • Human lung carcinoma cell line A549 isolated nuclei.
        Jang, S.H. et al. (2015) J. Biol. Chem. 290, 12547.
      • Mouse cerebral cortex.
        Duque, A. et al. (2013) Develop. Neurobiol. 73, 841.
      • Human lymphomas (1:70).
        Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
      • Human acute T-lymphoblastic lymphomas (1:300).
        Sanchez Miguel, D.S. et al. (2013) Biochem. Biophys. Res. Commun. 434, 273.
      • Mouse retina (1:200).
        Cangiano, L. et al. (2007) PLoS ONE 2, 1327.
      • HEK 293 transfected cells.
        Jimenez-Perez, L. et al. (2016) J. Biol. Chem. 291, 3569.
      • Human umbilical vascular endothelial cells (HUVECs) (1:100).
        Yang, L. et al. (2013) Toxicol. Lett. 223, 16.
      • Mouse leukaemia monocyte macrophage cells (raw 264.7), human Jurkat T-cells, human Raji B-lymphocytes.
        Vallejo Gracia, A. et al. (2013) J. Leukoc. Biol. 94, 779.
      • Human B-cell lymphoma cells (Raji cells), (1:100).
        Kawano, T. et al. (2009) Biol. Pharm. Bull. 32, 345.
      • Human T cells.
        Beeton, C. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 17414.
      • Hajdu, P. et al. (2015) Mol. Biol. Cell 26, 1640.
      • Gazula, V-R. et al. (2010) J. Comp.Neurol. 518, 3205.
      • Vicente, R. et al. (2008) J. Biol. Chem. 283, 8756.
    Related Products
        • Anti-KV1.3 (KCNA3) Antibody (#APC-002). This antibody recognizes a different epitope and thus can also be used as a control for detecting KV1.3.