Overview
Cat #:
APR-021-F
Alternative Name P2Y purinoceptor 1, ADP Receptor, P2RY1
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
- Peptide SDEYLRSYFIYSMC, corresponding to amino acid residues 207-220 of human P2RY1 (Accession P47900). Extracellular, 2nd loop.
Accession (Uniprot) Number P47900
Gene ID P2RY1
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rat - identical; mouse – 12 out of 14 amino acid residues identical.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG
Label Fluorescein isothiocyanate (FITC)
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-021), and direct flow cytometry (labeled antibody).
Applications: fc
May also work in: ic*, lci*
Direct flow cytometry
Cell surface detection of P2Y1 Receptor by direct flow cytometry in live intact mouse J774 macrophage cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC
___ Cells + Anti-P2Y1 Receptor (extracellular)-FITC Antibody (APR-021-F), 2.5µg.
Cell surface detection of P2Y1 Receptor by direct flow cytometry in live intact human MEG-01 megakaryoblastic leukemia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC
___ Cells + Anti-P2Y1 Receptor (extracellular)-FITC Antibody (APR-021-F), 2.5µg.
Cell surface detection of P2Y1 Receptor by direct flow cytometry in live intact mouse BV-2 microglia cells:___ Cells.
___ Cells + Rabbit IgG isotype control-FITC
___ Cells + Anti-P2Y1 Receptor (extracellular)-FITC Antibody (APR-021-F), 5µg.
Scientific background
The P2Y receptors belong to the G-protein coupled receptors superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiologic responses, among them, cardiac function, platelet aggregation and SMC proliferation.1
The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.2-4
P2Y1 receptor is abundantly expressed in a number of tissues. In platelets, P2Y1 receptor is co-expressed with P2Y12 receptor, responsible for the initial shape change, aggregation, and rise in intracellular Ca2+ upon activation in response to ADP.5
Lyophilized Powder
For research purposes only, not for human use
Last Update: 10/04/2023
Specifications
Citations
Citations
