Anti-P2Y2 Receptor (extracellular)-FITC Antibody

P2Y purinoceptor 2, P2RY2, P2U1
  • New
Cat #: APR-102-F
  • Lyophilized Powder
  • Antigen Incl.
  • Shipped at Room Temp.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
    Immunogen
    Peptide (CD)TINGTWEGDELGYK, corresponding to amino acid residues 11- 24 of rat P2RY2 (Accession P41232). Extracellular, C-terminus.
    Accession (Uniprot) Number P41232
    Gene ID 29597
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Rat, mouse – 14/15 amino acid residues identical; human – 13/15 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Formulation Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Label Fluorescein isothiocyanate (FITC).
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for two weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-102), and direct flow cytometry (labeled antibody).
    Applications: fc
    May also work in: ic, lci
    Direct flow cytometry
    Direct flow cytometry of live intact mouse J774 macrophage cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F), (2.5 µg).
    Direct flow cytometry of live intact human THP-1 monocytic leukemia cells:
    ___ Cells.
    ___ Cells + rabbit IgG isotype control-FITC.
    ___ Cells + Anti-P2Y2 Receptor (extracellular)-FITC Antibody (#APR-102-F), (5 µg).
    References
    1. Wang, L. et al. (2002) J. Cardiovas. Pharmacol40, 841.
    2. Queiroz, G. et al. (2003) J. Pharmacol. Exp. Ther307, 809.
    3. Ralevic, V. and Burnstock, G. (1998) Pharmacol. Rev50, 413.
    4. Abbracchio, M.P. et al. (2003) Trends Pharmacol. Sci24, 52.
    5. Di Virgilio, F. et al. (2001) Blood 97, 587.
    6. Yerxa, B.R. et al. (2002) J. Pharmacol. Exp. Ther302, 871.
    Scientific background

    P2Y receptors belong to the G-protein coupled receptor superfamily. P2Y receptors mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDP) and regulate several physiological responses, among them, cardiac function, platelet aggregation and SMC proliferation1.

    The mammalian P2Y family currently includes eight functional receptors; P2Y1, P2Y2, P2Y4P2Y6P2Y11P2Y12P2Y13, and the UDP-glucose receptor, now renamed P2Y142-4.

    The P2Y2 receptor is abundantly expressed in the apical membranes of several epithelial tissues including airway5. It has become a leading target for the treatment of cystic fibrosis. Activation of P2RY2 increases Cl- efflux and mucociliary clearance, effects that might relieve symptoms of cystic fibrosis. P2Y2 agonist therapy is tested in an attempt to bypass the dysfunctioning CFTR channel. Several P2Y2 receptor agonists, especially INS37217, being evaluated as possible targets for the treatment of cystic fibrosis6.

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 06/11/2018

    Anti-P2Y2 Receptor (extracellular) Antibody (#APR-102) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize P2RY2 from rat, mouse, and human samples.

    Anti-P2Y2 Receptor (extracellular)FITC Antibody (#APR-102-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

    For research purposes only, not for human use
    Citations
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