Overview
- Peptide CVKDYDLERGPDEK, corresponding to amino acid residues 802-815 of mouse TRPM6 (Accession Q8CIR4). 1st extracellular loop.
- Rat kidney, testis and mouse kidney lysates (1:200).
- Western blot analysis of rat kidney (lanes 1 and 4), rat testis (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates:1-3. Anti-TRPM6 (extracellular) Antibody (#ACC-046), (1:200).
4-6. Anti-TRPM6 (extracellular) Antibody, preincubated with TRPM6 (extracellular) Blocking Peptide (#BLP-CC046).
- Mouse placenta sections (1:50) (Suzuki, Y. et al. (2016) J. Physiol. Sci. 67, 151.).
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3
According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and TRPM (melastatin). To date, three extra subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4
The TRPM subfamily consists of eight members, TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their sequence homology: (1)TRPM1 and TRPM3 (2) TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5