Name: Anti-CACNA1G (CaV3.1)-ATTO Fluor-594 Antibody
Brand: Alomone Labs
SKU: ACC-021-AR
Price: 686.00 USD
Availability: InStock

Cat #: ACC-021-AR

Alternative Name Voltage-dependent T-type calcium channel subunit α_1G

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide MDEEEDGAGAEESGQPRSFTQL(C), corresponding to amino acid residues 1-22 of rat CACNA1G (Accession O54898). Intracellular, N-terminus.

Accession (Uniprot) Number O54898

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse - identical; human - 20/22 amino acid residues identical.

RRID AB_2340896.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Isotype Rabbit IgG.

Label ATTO-594. Maximum absorption 601 nm; maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label is related to the Rhodamine dyes and can be used with filters used to detect Texas Red and Alexa-594.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 50 µl double distilled water (DDW).

Antibody concentration after reconstitution 1 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

Standard quality control of each lot Western blot analysis (unlabeled antibody, #ACC-021), and immunohistochemistry (labeled antibody).

Applications: if, ih

May also work in: ic*

Immunohistochemistry

Rat and mouse cerebellum.
Expression of CACNA1G in rat and mouse cerebellum
Immunohistochemical staining of mouse (A) and rat (B) cerebellum using Anti-CACNA1G (Ca_V3.1)-ATTO Fluor-594 Antibody (#ACC-021-AR). A, B. CACNA1G staining (red) appears in Purkinje neurons (arrow) and in the molecular layer (Mol). Nuclei were stained using DAPI as the counterstain (Blue).

Scientific background

Voltage-dependent Ca²⁺ channels provide a pathway for rapid influx of Ca²⁺ into cells, which plays a crucial role in both electrical and metabolic signaling.¹

T-type currents are transduced via channel proteins encoded by three genes that compose a subfamily within the Ca_V channel family.^2-3

The activity of T-type channels contributes to several known physiological and pathophysiological phenomena including burst firing in neurons, pacemaking activity in the heart and secretion from endocrine tissues.² There are three cloned T-type channel isoforms.

CACNA1G (Ca_V3.1) and CACNA1H (Ca_V3.2) are widely distributed whereas the expression of CACNA1I (Ca_V3.3) is restricted to the central nervous system.²

CACNA1G and CACNA1H are also expressed in the kidney, but little is known about their physiological role there.

Lyophilized Powder

Last update: 11/04/2021

For research purposes only, not for human use

Specifications

Applications

Scientific Background

Citations

Shipping and Ordering information

Control Product

A Blocking peptide for Anti-CACNA1G (Ca_V3.1) Antibody (#ACC-021) and Anti-CACNA1G (Ca_V3.1)-ATTO Fluor-594 Antibody (#ACC-021-AR). Intended to be used as a negative control.

Price: $245

CACNA1G/Cav3.1 Blocking Peptide (#BLP-CC021)
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Certificate of Analysis

Anti-CACNA1G (Ca_V3.1)-ATTO Fluor-594 Antibody

Application key:

Species reactivity key:

Specifications

Applications

Scientific Background

Citations

Resources

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