Anti-Human CaV1.2 (CACNA1C) Antibody

Human α1C
    Cat #: ACC-022
    Alternative Name Human α1C
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Host: Rabbit
    Reactivity: h, m, r
    • Peptide VNENTRMYIPEENHQ(C), corresponding to amino acid residues 2-15 of human CaV1.2 (exon 1B) (Accession Q13936). Intracellular, N-terminus.
    • Anti-Human CaV1.2 (CACNA1C) Antibody
    Accession (Uniprot) Number Q13936
    Gene ID 775
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse, rat - 14/15 amino acid residues identical.
    RRID AB_2039769.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Specificity The antibody recognizes mouse CaV1.2, splice variants P22002-4 and P22002-5 of rat CaV1.2 and splice variant P15381-4 of rabbit CaV1.2.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 25 μl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.8 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Negative control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Negative control antigen reconstitution 100 µl double distilled water (DDW).
    Negative control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, if, wb
    May also work in: ifc*, ih*, ip*
    Western blot
    • Anti-Human CaV1.2 (CACNA1C) Antibody
      Western blot analysis of rat heart membranes:
      1. Anti-Human-CaV1.2 (CACNA1C) Antibody (#ACC-022), (1:200).
      2. Anti-Human-CaV1.2 (CACNA1C) Antibody, preincubated with the negative control antigen.
    • Mouse cortex, hippocampus, and cerebellum lysates (1:200) (White, J.A. et al. (2008) Learn. Mem. 15, 1.).
    1. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 579.
    2. IUPHAR
    Scientific background

    All L-type calcium channels are encoded by one of the CaV1 channel genes. These channels play a major role as a Ca2+ entry pathway in skeletal, cardiac and smooth muscles as well as in neurons, endocrine cells and possibly in non-excitable cells such as hematopoetic and epithelial cells. All CaV1 channels are influenced by dihydropyridines (DHP) and are also referred to as DHP receptors.

    While the CaV1.1 and CaV1.4 isoforms are expressed in restricted tissues (skeletal muscle and retina, respectively), the expression of CaV1.2 is ubiquitous and CaV1.3 channels are found in the heart, brain and pancreas. Several peptidyl toxins are described that are specific L-type channel blockers, but so far no selective blocker for one of the CaV1 isoforms have been described. These include the Mamba toxins Calcicludine (#SPC-650), Calciseptine (#C-500) and FS-2 (#F-700).

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 24/01/2020

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human CaV1.2 channel. Anti-Human CaV1.2 (CACNA1C) Antibody (#ACC-022) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CaV1.2 from mouse, rat and human samples.

    For research purposes only, not for human use



    Scientific Background


    Western blot citations
    1. Mouse cortex, hippocampus, and cerebellum lyastes (1:200).
      White, J.A. et al. (2008) Learn. Mem. 15, 1.
    Immunocytochemistry citations
    1. Rat nodose ganglion neurons in culture (1:15).
      Pamidimukkala, J. and Hay, M. (2004) Brain Res. 1006, 215.
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