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Anti-KCNE2 (MiRP1) Antibody

Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2

Cat #: APC-054
Alternative Name Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2 (Accession P63161). Intracellular, C-terminal domain.
Accession (Uniprot) Number P63161
Gene ID 171138
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical; rabbit - 15/20 amino acid residues identical.
RRID AB_2039925.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody concentration after reconstitution 0.6 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Standard quality control of each lot Western blot analysis.
Applications: ic, if, ih, ip, wb
May also work in: ifc*
Western blot
  • Western blot analysis of rat heart membranes:
    Western blot analysis of rat heart membranes:
    1. Anti-KCNE2 (MiRP1) Antibody (#APC-054), (1:200).
    2. Anti-KCNE2 (MiRP1) Antibody, preincubated with KCNE2/MiRP1 Blocking Peptide (#BLP-PC054).
  • Human ventricle lysate (Zhang, M. et al. (2012) Am. J. Physiol302, H910.).
Immunoprecipitation
  • Rat sinoatrial cells (SANs) (Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.).
Immunohistochemistry
  • Expression of KCNE2 in mouse heart
    Expression of KCNE2 in mouse heart
    Immunohistochemical staining of mouse heart frozen sections using Anti-KCNE2 (MiRP1) Antibody (#APC-054), (red). Immunoreactivity appears as a striated pattern in heart muscle.
Immunocytochemistry
  • Rabbit carotid body chemoreceptor cells (Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.).
References
  1. Abbott, G.W. et al. (1999) Cell 97, 175.
  2. Abbott, G.W. and Goldstein S.A. (2001) Mol. Interv. 1, 95.
  3. Roepke, T.K. et al. (2006) J. Biol. Chem. 281, 23740.
Scientific background

KCNE2 (or MiRP1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE5 (MiRP4).

KCNE1 is the founding member of the family and KCNE2 was discovered based on its homology with KCNE1.1,2

The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.1,2

KCNE2 is broadly distributed with prominent expression in the brain and heart, but also in other tissues such as skeletal muscle, pancreas and kidney.

The best described functional association of KCNE2 is with the voltage-gated K+ channel KV11.1 (HERG). In the heart, complexes of the two proteins constitute the molecular underlay of the cardiac IKr current and mutations in the KCNE2 subunit are associated with cardiac arrhythmias.1 KCNE2 can form complexes with other channels including the K+ channels KV7.1 (KCNQ1) and KV4.3 and the hyperpolarization-activated cation channels HCN1 and HCN2. In the stomach KV7.1/KCNE2 complexes were found to be crucial for acid secretion as well as for the maintenance of structural integrity, and for parietal cell survival.3

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Last update: 11/04/2021

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNE1. Anti-KCNE2 (MiRP1) Antibody (#APC-054) can be used in western blot, immunohistochemistry, immunocytochemistry and immunoprecipitation applications. It has been designed to recognize KCNE2 from rat, mouse and human samples.

For research purposes only, not for human use

Applications

Specifications

Scientific Background

Citations

Citations
Western blot citations
  1. Rat ventricular myocytes (1:200).
    Liu, W.J. et al. (2015) Am. J. Physiol. 309, H1288.
  2. Cultured mouse primary cortical/hippocampal neurons (1:250).
    Sachse, C.C. et al. (2013) FASEB J. 27, 2458.
  3. Human ventricle lysate.
    Zhang, M. et al. (2012) Am. J. Physiol. 302, H910.
Immunoprecipitation citations
  1. Rat sinoatrial cells (SANs).
    Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.
Immunocytochemistry citations
  1. Rabbit carotid body chemoreceptor cells.
    Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.
More product citations
  1. Wang, Y. et al. (2012) J. Gen. Physiol. 140, 653.
  2. Jiang, M. et al. (2004) Circulation 109, 1783.
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