Anti-KCNE2 (MiRP1) Antibody

Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2
Cat #: APC-054
Alternative Name Potassium channel subunit β MiRP1, MinK-related peptide 1, Potassium voltage-gated channel subfamily E member 2
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)IVEDWQQKYRSQILHLEDSK, corresponding to amino acid residues 88-107 of rat KCNE2 (Accession P63161). Intracellular, C-terminal domain.
    Accession (Uniprot) Number P63161
    Gene ID 171138
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical; rabbit - 15/20 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
    Isotype Rabbit IgG.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.
    Antibody concentration after reconstitution 0.6 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 µg peptide per 1 µg antibody.
    Standard quality control of each lot Western blot analysis.
    Applications: ic, ih, ip, wb
    May also work in: ifc
      • Western blot analysis of rat heart membranes:
        1. Anti-KCNE2 (MiRP1) Antibody (#APC-054), (1:200).
        2. Anti-KCNE2 (MiRP1) Antibody, preincubated with the control peptide antigen.
        Human ventricle lysate (Zhang, M. et al. (2012) Am. J. Physiol302, H910.).
      • Rat sinoatrial cells (SANs) (Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.).
      • Expression of KCNE2 in mouse heart
        Immunohistochemical staining of mouse heart frozen sections using Anti-KCNE2 (MiRP1) Antibody (#APC-054), (red). Immunoreactivity appears as a striated pattern in heart muscle.
      • Rabbit carotid body chemoreceptor cells (Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.).
      • KCNE2 (or MiRP1) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE3 (MiRP2), KCNE4 (MiRP3) and KCNE5 (MiRP4).

        KCNE1 is the founding member of the family and KCNE2 was discovered based on its homology with KCNE1.1,2

        The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.1,2

        KCNE2 is broadly distributed with prominent expression in the brain and heart, but also in other tissues such as skeletal muscle, pancreas and kidney.

        The best described functional association of KCNE2 is with the voltage-gated K+ channel KV11.1 (HERG). In the heart, complexes of the two proteins constitute the molecular underlay of the cardiac IKr current and mutations in the KCNE2 subunit are associated with cardiac arrhythmias.1 KCNE2 can form complexes with other channels including the K+ channels KV7.1 (KCNQ1) and KV4.3 and the hyperpolarization-activated cation channels HCN1 and HCN2. In the stomach KV7.1/KCNE2 complexes were found to be crucial for acid secretion as well as for the maintenance of structural integrity, and for parietal cell survival.3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Last update: 17/06/2019

    Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human KCNE1. Anti-KCNE2 (MiRP1) Antibody (#APC-054) can be used in western blot, immunohistochemistry, immunocytochemistry and immunoprecipitation applications. It has been designed to recognize KCNE2 from rat, mouse and human samples.

    For research purposes only, not for human use
    Citations
      • Rat ventricular myocytes (1:200).
        Liu, W.J. et al. (2015) Am. J. Physiol. 309, H1288.
      • Cultured mouse primary cortical/hippocampal neurons (1:250).
        Sachse, C.C. et al. (2013) FASEB J. 27, 2458.
      • Human ventricle lysate.
        Zhang, M. et al. (2012) Am. J. Physiol. 302, H910.
      • Rat sinoatrial cells (SANs).
        Qu, J. et al. (2004) J. Biol. Chem. 279, 43497.
      • Rabbit carotid body chemoreceptor cells.
        Colinas, O. et al. (2008) J. Gen. Physiol. 131, 455.
      • Wang, Y. et al. (2012) J. Gen. Physiol. 140, 653.
      • Jiang, M. et al. (2004) Circulation 109, 1783.
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