Anti-P2X7 Receptor-ATTO-550 Antibody

Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
Cat #: APR-004-AO
Alternative Name Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
  • KO Validated
  • Lyophilized Powder
  • Antigen Incl.
  • Type: Polyclonal
    Source: Rabbit
    Reactivity: h, m, r
      • Peptide (C)KIRKEFPKTQGQYSGFKYPY, corresponding to amino acid residues 576-595 of rat P2X7 receptor (Accession Q64663). Intracellular, C-terminus.
    Accession (Uniprot) Number Q64663
    Gene ID 29665
    Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
    Homology Mouse - 18/20 amino acid residues identical; human - 16/20 amino acid residues identical.
    Purity Affinity purified on immobilized antigen.
    Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.025% NaN3.
    Isotype Rabbit IgG.
    Label ATTO-550. Maximum absorption 554 nm; maximum fluorescence 576 nm. The fluorescence is excited most efficiently in the 540 - 565 nm range. This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine.
    Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
    Reconstitution 50 µl double distilled water (DDW).
    Antibody concentration after reconstitution 1 mg/ml.
    Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
    Control antigen storage before reconstitution Lyophilized powder can be stored intact at room temperature for 2 weeks. For longer periods, it should be stored at -20°C.
    Control antigen reconstitution 100 µl double distilled water (DDW).
    Control antigen storage after reconstitution -20°C.
    Preadsorption Control 1 μg peptide per 1 μg antibody.
    Standard quality control of each lot Western blot analysis (unlabeled antibody, #APR-004), and immunohistochemistry (labeled antibody).
    Applications: ih
    May also work in: ic
      • Expression of P2RX7 in rat hippocampus
        Immunohistochemical staining of rat hippocampus frozen section using Anti-P2X7 Receptor Antibody (#APR-004-AO), (1:60). A. P2X7 staining (red) appears in the CA1 pyramidal (Pyr) and stratum radiatum  (SR) layers in cells with glial morphology (arrows). B. DAPI is used as the counterstain (blue). C. Merged image of A and B.
        Immuno-colocalization of VGLUT2 and P2X7 Receptor in rat spinal cord
        Immunohistochemical staining of perfusion-fixed frozen rat spinal cord sections using Anti-VGLUT2 Antibody (#AGC-036), (1:600) and Anti-P2X7 Receptor-ATTO-550 Antibody (#APR-004-AO), (1:100). A. Vesicular Glutamate Transporter 2 labeling followed by goat-anti-rabbit-Alexa-488 (green). B. The same section labeled for P2X7 Receptor (orange). C. Merge of A and B demonstrates partial co-localization of VGLUT2 and P2X7 Receptor in dorsal horn and in lateral column (L. Col., arrow). Cell nuclei were stained with DAPI (blue).
        Expression of P2X7 Receptor in rat pancreas
        Immunohistochemical staining of rat paraffin embedded endocrine and exocrine pancreas sections using Anti-P2X7 Receptor-ATTO-550 Antibody (#APR-004-AO), (1:20), (red). Staining is highly specific for endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain (blue).
        Mouse spinal cord sections (1:20) (Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102.).
      • The P2X7 purinergic receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family consists of seven receptor subtypes, named P2X1-P2X7, all of which have been cloned.

        The various P2X receptors show distinct expression patterns. P2X1-6 receptors have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly in antigen-presenting cells, and microglia.

        The P2X7 receptor mediates the release of proinflammatory cytokines and stimulation of transcription factors and may also play an important role in apoptosis.1-3

    Application key:

    CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

    Species reactivity key:

    H- Human, M- Mouse, R- Rat
    Image & Title: Anti-P2X7 Receptor-ATTO-550 Antibody
    Expression of P2X7 purinergic receptor in mouse spinal cordImmunohistochemical staining of mouse spinal cord sections (L3–L5) from SOD1-G93A mice were double-immunostained either with anti-Iba-1, NeuN or GFAP (green) and Anti-P2X7 Receptor-ATTO-550 Antibody (#APR-004-AO), (red). P2X7 is present only in microglia cells as shown in the merged panel (yellow). Scale bar = 20 μm, insets = 50 μm.Adapted from Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102. with permission of Oxford University Press.
    Last update: 11/06/2019

    Anti-P2X7 Receptor Antibody (#APR-004) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2X7 purinergic receptor from rat, mouse, and human samples.

    Anti-P2X7 Receptor-ATTO-550 Antibody (#APR-004-AO) is directly labeled with an ATTO-550 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Anti-P2X7 Receptor-ATTO-550 Antibody has been tested in immunohistochemistry applications and is specially suited to experiments requiring simultaneous labeling of different markers.

    For research purposes only, not for human use
    Citations
      • Western blot analysis of mouse neutrophil lysate using #APR-004. Tested in P2X7-/- mice.
        Karmakar, M. et al. (2016) Nat. Commun. 7, 10555.
      • Western blot analysis of mouse GL261 glioma cell lysate using #APR-004. Tested on siRNA treated cells.
        Gehring, M.P. et al. (2015) Int. J. Biochem. Cell Biol. 68, 92.
      • Immunocytochemical staining of mouse microglial cells with #APR-004 (1:600). Tested on P2X7-/- cells.
        Fischer, W. et al. (2014) Purinergic Signal. 10, 313.
      • Western blot analysis of mouse lumbar spinal cord protein lysates using #APR-004 (1:500). Tested in P2X7-/- mice.
        Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102.
      • Rat brain sections.
        Kuan, Y.H. et al. (2015) Neurobiol. Dis. 78, 134.
      • Mouse spinal cord sections (1:20).
        Apolloni, S. et al. (2013) Hum. Mol. Genet. 22, 4102.
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