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- 5-10 μl antibody/1x106 cells.Cell surface detection of P2RX7 in live intact human THP-1 monocytic leukemia cells:___ Cells.
___ Cells + rabbit IgG isotype control-FITC.
___ Cells + Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F), (5 µg).Cell surface detection of P2RX7 in live intact living Jurkat T-cells:___ Unstained cells.
___ Cells + Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F), (10 µg per 1x106 cells).The control antigen is not suitable for this application.
- Human live cells, K562 (1:10).
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7.
The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflamatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3
Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
Species reactivity key:
Immuno-colocalization of P2X7 receptor and cholera toxin in MC3T3-E1 cells.Immunocytochemical staining with labeled cholera toxin and Anti-P2X7 Receptor (extracellular)-FITC Antibody (#APR-008-F). The ganglioside GM1, the receptor for cholera toxin is concentrated in caveolae. Staining shows that P2X7 receptor colocalizes with cholera toxin.
Adapted from Gangadharan, V.et al. (2014) with permission of the American Physiological Society.