QX-314 chloride

Lidocaine N-ethyl chloride
A Local Anesthetic Blocker of NaV Channels
    Cat #: Q-150
    Alternative Name Lidocaine N-ethyl chloride
  • Lyophilized Powder
  • Bioassay Tested
  • Source Synthetic
    MW: 298.85
    Purity: >99% (HPLC)
    Effective concentration 1-50 mM.
      • QX-314 chloride
    Chemical name N-(2,6-Dimethylphenylcarbamoylmethyl) triethylammonium chloride.
    Molecular formula C16H27N2OCl.
    CAS No.: 5369-03-9.
    Activity QX-314 chloride is an intracellular blocker of NaV channels1-4.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for four years.
    Solubility Water. Centrifuge all product preparations before use (10000 x g 5 min).
    Storage of solutions Up to two weeks at 4°C or six months at -20°C.
    Protect from light. Hygroscopic.
      • QX-314 chloride
        Alomone Labs QX-314 chloride inhibits native NaV1.5 currents expressed in Xenopus oocytes.
        Dose-response of QX-314 chloride (#Q-150) inhibition on inward Na+ currents. Using two electrode voltage clamps, membrane potential was held at -100 mV and currents were elicited by 25 ms depolarization to -20 mV, delivered every 5 sec. Left: Dose-response curve for one experiment. Right: Traces before (red) and during bath application of different concentrations of QX-314 chloride.
    References - Scientific background
      • This local anesthetic is a blocker of voltage-dependent Na+ channels. QX-314 blocks all NaV channels once exposed to the intracellular face of the channel protein. However, this compound can penetrate the cell interior once applied outside the cell. Via certain types of NaV channels, it can permeate and exert blocking activity via TTX-sensitive channels.1 Intracellular recordings from inferior olivary neurons in brain stem slice preparations demonstrated that Na+-dependent action potentials were completely blocked for approximately five minutes after impalement with a microelectrode containing 50 mM QX-314, whereas the low-threshold Ca2+ spike was still generated.2 QX-314 is widely used to block NaV channels and used as a tool in neurophysiological recordings.2-5
    Target Various NaV Na+ channels
    Last update: 24/01/2020

    QX-314 chloride (#Q-150) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use
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