Cat #: T-650
This product is freeze dried. All water molecules have been removed.
Every lot is tried & tested in a relevant biological assay.
Origin Thapsia garganica.
Purity: >99% (HPLC)
Effective concentration 50 nM - 1 μM.
Chemical name (3S,3aR,4S,6S,6AR,7S,8S,9bS)-6-(Acetyloxy)-2,3,3a,4,5,6 ,6a,7,8,9b-decahydro-3,3a-dihydroxy-3,6,9-trimethyl-8-[ [(2Z)-2-methyl-1-oxo-2-butenyl]oxy]-2-oxo-4-(1-oxobutox y)azuleno[4,5-b]furan-7-yl octanoate.
Molecular formula C34H50O12.
CAS No.: 67526-95-8.
Activity Thapsigargin is a highly potent inhibitor of the sarco-endoplasmic reticulum Ca2+-ATPases (SERCAs), inhibiting Ca2+ pumping to intracellular stores at nanomolar concentrations, with IC50 ranging from 0.2 nM to 50 nM in various mammalian cell types.1 Thapsigargin-induced depletion of Ca2+ stores leads to apoptosis in various cell lines.2-4
Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
Solubility DMSO, ethanol or acetone. Centrifuge all product preparations before use (10000 x g 5 min).
Storage of solutions Up to one week at 4°C or three months at -20°C.
- Alomone Labs Thapsigargin induces cytosolic Ca2+ increase in Jurkat cells.Cells were loaded with Fluo-3-AM dye in the presence of 5 mM EGTA. 20 nM Thapsigargin (#T-650) application induces a significant increase in intracellular Ca2+. Changes in intracellular Ca2+ were detected as changes in Fluo-3 fluorescent emission following the application (arrow) of control or Thapsigargin.Alomone Labs Thapsigargin induces apoptosis in Jurkat cells.Cells were grown to 70% confluency. 1 μM Thapsigargin (#T-650) or vehicle was added for six hours. Cell extracts were then probed for cleaved Caspase 3 with specific antibodies.Alomone Labs Thapsigargin increases intracellular Ca2+ by modulating the autophosphorylation of CaMK II in 3T3-L1 cells.Cells were starved for 2h and then stimulated with 1 or 5 µM Thapsigargin (#T-650) for the indicated times. The cell extracts were blotted and probed with an antibody for phospho-(Thr268)-CAMKII (Calmodulin dependent kinase II).Alomone Labs Thapsigargin induces cytosolic Ca2+ increase in HEK 293 cells.Ca2+ traces from Fluo-3 AM loaded HEK 293 cells treated with 5 µM Thapsigargin (#T-650) in the presence of 5 mM EGTA.
References - Scientific background
- 1. Treiman, M. et al. (1998) Trends Pharmacol. Sci. 19, 131.
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- Thapsigargin, derived from the plant genus Thapsia,1-3 is an extremely tight-binding inhibitor of intracellular Ca2+ pumps. It was initially described as a tumor promoting agent which induces rapid Ca2+ release from intracellular stores4 by inhibition of the sarcoplasmic/endoplasmic reticulum Ca2+-dependent ATPase pump without inositol phosphate formation.5-7 The thapsigargin induced depletion of Ca2+ stores causes apoptosis in most cell lines.8-10 It has also been shown to cause histamine secretion from rat mast cells,11 and to stimulate arachidonic acid metabolism in macrophages.12 Its tumour-promoting function probably results, at least partly, from cytotoxicity, causing a wound response in the skin.13 The tumorogenic activity of thapsigargin might be due to its activation of protein kinase B (Akt) which subsequently stimulates MAP kinase signaling via Src and Raf-1.12,13
Image & Title Alomone Labs Thapsigargin decreases Ca2+ signals in rat mossy fibers.Rat hippocampal slices were incubated with 10 µM Thapsigargin (#T-650) for 60 min. The Ca2+ signals from mossy ﬁber terminals decreased signiﬁcantly after wash-in.Adapted from Liang, Y. et al. (2002) J. Neurophysiol. 87, 1132. with permission of The American Physiological Society.
Last update: 21/11/2019
Thapsigargin (#T-650) is a highly pure, natural and biologically active compound.
For research purposes only, not for human use
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