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Naspm trihydrochloride

An Antagonist of AMPA Receptors

Cat #: N-215
Lyophilized Powder yes
  • Bioassay Tested
  • Source Synthetic
    MW: 479.91
    Purity: >95% (HPLC)
    Form Lyophilized powder.
    Effective concentration 300 nM - 10 µM.
    Chemical name N-[3-[[4-[(3-aminopropyl)amino]butyl]amino]propyl]-1-naphthaleneacetamide trihydrochloride.
    Molecular formula C22H37Cl3N4O.
    CAS No.: 1049731-36-3
    Activity Naspm trihydrochloride is a selective AMPA receptor antagonist; ~0.3 uM IC50 for AMPA induced currents in rat hipocampal neurons1.
    1. Koike, M. et al. (1997) Neurosci. Res29, 27.
    Shipping and storage Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
    Solubility 50 mM in H2O. Centrifuge all product preparations before use (10000 x g for 1 min).
    Storage of solutions Up to four weeks at 4°C or three months at -20°C.
    Our bioassay
    • Alomone Labs Naspm trihydrochloride inhibits GluA1 channels expressed in Xenopus oocytes.
      Alomone Labs Naspm trihydrochloride inhibits GluA1 channels expressed in Xenopus oocytes.
      Time course of current reversible inhibition by 0.2, 1 and 5 μM Naspm trihydrochloride (#N-215) of glutamate-elicited currents (100 μM, holding potential -60 mV).
    References - Scientific background
    1. Koike, M. et al. (1997) Neurosci. Res29, 27.
    Scientific background

    1-Naphthyl acetyl spermine (NASPM), a synthetic analogue of Joro spider toxin (JSTX), is a selective antagonist of Ca2+-permeable AMPA receptors. It blocks AMPA receptors lacking the GluR2 subunit expressed in rat hippocampal neurons with IC50 values of 0.33 μM1.

    NASPM has been shown to block excitatory synaptic potentials evoked by stimulation of parallel fibers in guinea pig cerebellar Purkinje cells. In the hippocampus, NASPM has been reported to exert a potent and selective suppression of hippocampal epileptic discharges mediated by non-NMDA receptors. In addition, it has been reported that EPSCs in CA1 neurons of the gerbil hippocampus after ischemia are mediated by Ca2+-permeable non-NMDA receptors, and that these abnormal EPSCs are effectively suppressed by NASPM.

    These data suggest that NASPM may be useful as a pharmacological tool for investigating the physiological and pathological roles of AMPA receptors, and furthermore, that it could be used as a parent compound to develop new agents for the treatment of neuronal cell death and epilepsy1.

    Target AMPA receptors
    Image & Title Naspm trihydrochloride
    Alomone Labs Naspm trihydrochloride reduces AMPA receptor-induced Ca2+ transients in isolated olfactory bulb astrocyte.Isolated mouse olfactory bulb astrocyte was treated with Naspm trihydrochloride (#N-215). On average, 25 μM AMPA induced Ca2+ transients with an amplitude of 2.50 ± 0.50 ΔF/F0 (n = 31), which were reduced to 52.7 ± 5.3% by Naspm trihydrochloride.Adapted from Droste, D. et al. (2017) Sci. Rep. 7, 44817. with permission of Nature publishing group.
    Last update: 06/11/2022

    Naspm trihydrochloride (#N-215) is a highly pure, synthetic, and biologically active compound.

    For research purposes only, not for human use



    1. Mouse olfactory bulb (whole-cell voltage clamp).
      Beiersdorfer, A. and Lohr, C. (2019) Front. Cell. Neurosci. 13, 451.
    Product citations
    1. Droste, D. et al. (2017) Sci. Rep. 7, 44817.


    Scientific Background

    Shipping and Ordering information