Centrifuge all products before use (10000 x, g 5 min). Avoid multiple freezing and thawing. It is recommended to prepare fresh solutions in working buffers before use, or aliquot stock solutions reconstituted in distilled water and keep at -20°C. Upon use, dilute the stock solution in the desired working buffer.
- Alomone Labs Anthopleurin-C enhances NaV1.5 channel currents expressed in HEK cells.Representative superimposed traces of NaV1.5 currents in the absence (black) or presence (green) of 10 nM Anthopleurin-C (#STA-400). Currents were elicited by a 20 ms voltage step to -20 mV every 3 sec from a holding potential of -90 mV.
The figure was kindly provided by Prof. Shai Berlin, Ruth and Bruce Rappaport Faculty of Medicine, Technion, Israel.
A potent cardiotoxin, which evokes a positive inotropic and chronotropic effect on mammalian heart muscle.1,2 This toxin was found to modify the current passing through the fast Na+ channel in neuroblastoma cells, leading to delay and incomplete inactivation.2
Effective at concentrations of 1-5 nM in isolated guinea-pig atria.2
Anthopleurin-C (#STA-400) is a highly pure, natural, and biologically active peptide toxin.