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Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody

Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA

Cat #: ASC-008-FR
Alternative Name Voltage-gated sodium channel type IX subunit alpha, PN1, NENA, NE, ETHA
Lyophilized Powder yes
Type: Polyclonal
Host: Rabbit
Reactivity: h, m, r
Immunogen
  • Peptide (C)EFTSIGRSR IMGLSE, corresponding to amino acid residues 446-460 of rat NaV1.7 (Accession O08562). Intracellular loop between domains I and II.
Accession (Uniprot) Number O08562
Gene ID 78956
Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.
Homology Rabbit - 14/15 amino acid residues identical; human, mouse - 13/15 amino acid residues identical.
RRID AB_2341066.
Purity Affinity purified on immobilized antigen.
Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Isotype Rabbit IgG.
Label ATTO-633. Maximum absorption 629 nm; maximum fluorescence 657 nm. The fluorescence is excited most efficiently in the 610 - 645 nm range. This label is analogous to the dyes Alexa 647, Alexa 633 and Cy5 and can be used for direct flow cytometry (FACS) using the He:Ne laser.
Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Reconstitution 50 µl double distilled water (DDW).
Antibody concentration after reconstitution 1 mg/ml.
Storage after reconstitution The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Standard quality control of each lot Western blot analysis (unlabeled antibody, #ASC-008), and immunohistochemistry (labeled antibody).
Applications: if, ih
May also work in: ic*
Immunohistochemistry
  • Expression of NaV1.7 in rat DRG
    Expression of NaV1.7 in rat DRG
    Immunohistochemical staining of rat dorsal root ganglion (DRG) using Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR). A. NaV1.7 staining (purple) appears in DRG neurons. B. Nuclear staining using DAPI as the counterstain. C. Merge images of A and B.
References
  1. Wu, L. et al. (2002) NeuroReport 13, 2547.
  2. Fang, X. et al. (2002) J. Neurosci. 22, 7425.
  3. Fjell, J. et al. (2000) NeuroReport 11, 199.
  4. Baker, M.D. and Wood, J.N. (2001) Trends Pharmacol. Sci. 22, 27.
  5. Lai, J. et al. (2003) Curr. Opin. Neurobiol. 13, 291.
  6. Isom, L.L. (2001) Neuroscientist 7, 42.
  7. Catterall, W.A. et al. (2003) Pharmacol. Rev. 55, 575.
  8. Catterall, W.A. et al. (2005) Pharmacol. Rev. 57, 397.
  9. Yang, Y. et al. (2004) J. Med. Genet. 41, 171.
  10. Cummins, T.R. et al. (2004) J. Neurosci. 24, 8232.
  11. Dray, A. (2008) Br. J. Anaesth. 101, 48.
Scientific background

Voltage-gated sodium channels (NaV) are essential for the generation of action potentials and for cell excitability1. NaV channels are activated in response to depolarization and selectively allow the flow of Na+ ions. To date, nine NaV α subunits have been cloned and named NaV1.1-NaV1.94-5. The NaV channels are classified into two groups according to their sensitivity to tetrodotoxin (TTX): TTX-sensitive (NaV1.1, NaV1.2, NaV1.3, NaV1.4, NaV1.6 and NaV1.7) and TTX-resistant (NaV1.5, NaV1.8 and NaV1.9)2-3.

Mammalian sodium channels are heterotrimers composed of a central, pore-forming α subunit and two auxiliary β subunits. The expression of the α subunit isoform is developmentally regulated and tissue specific. Na+ channels in the adult central nervous system and heart contain β1 through β4 subunits, whereas Na+ channels in adult skeletal muscle have only the β1 subunit6,8.

NaV1.7 is predominantly expressed in dorsal root ganglions (DRG) of the peripheral nervous system. Dominant gain of function mutations in the NaV1.7 gene are associated with erythermalgia (a rare autosomal disease characterized by sporadic burning pain accompanied by redness and heat in the extremities).9-11 Loss of function mutations in NaV1.7 channels leads to complete ablation of pain perception in humans.11 These recent findings highlight the role of this NaV isoform and the subset of DRG neurons that express this channel in physiological pain sensation.

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat
Lyophilized Powder
Last update: 08/06/2022

Anti-NaV1.7 (SCN9A) Antibody (#ASC-008) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize NaV1.7 from rat, human, and mouse samples.

Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-NaV1.7 (SCN9A)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.

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For research purposes only, not for human use

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