Name: µ-Conotoxin SxIIIC
Brand: Alomone Labs
SKU: STC-130

Overview

Cat #: STC-130

Alternative Name Mu-conotoxin SxIIIC, SxIIIC peptide

Lyophilized Powder yes

Origin Conus striolatus (Cone snail)

Source Synthetic peptide

MW: 2437 Da

Purity: >98%

Form Lyophilized Powder

Effective concentration 15 – 200 nM

Sequence RGCCNGRGGCSSRWCRDHARCC-NH₂

Modifications Disulfide bonds location - Cys³-Cys¹⁵, Cys⁴-Cys²¹ and Cys¹⁰-Cys²²Cys22 - Cysteine amide

Structure

Molecular formula C₉₀H₁₄₁N₄₁O₂₈S₆

Activity Mu-conotoxin SxIIIC most potently blocks Na_V1.4 channel, with 10-fold selectivity over Na_V1.1, 1.3, 1.6 and 1.7, 24-fold selectivity over hNa_V1.2.

References-Activity

McMahon, K.L. et al. (2020) Biomedicines, 8, 321.

Accession number A0A7M4DUE8

Shipping and storage The product is shipped as a lyophilized powder at room temperature. Upon receipt, it should be stored at -20°C. Protect from moisture.

Solubility Soluble in water. For long-term storage in solution, we recommend to prepare a stock solution by dissolving the product in ddH2O at a concentration X100-1000 of final working solution. Divide the solution into small aliquots and store at -20°C. Upon use, thaw the relevant vial intended for use and dilute in your desired working buffer. The preparation of fresh solutions in working buffers before use is recommended. Repeat freeze-thawing might result in loss of activity. Centrifuge all product preparations before use (10000 x g, 5 min).

Storage of solutions The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the toxin in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Our bioassay

Alomone Labs μ-Conotoxin SxIIIC inhibits Na_V1.4 channel currents heterologously expressed in Xenopus oocytes.
A. Representative time course of μ-Conotoxin SxIIIC (#STC-130) inhibition of Na_V1.4 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage step to 0 mV every 10 sec, and significantly inhibited by application of 50 nM μ-Conotoxin SxIIIC (green).
B. Superimposed traces of Na_V1.4 channel currents in the absence (control) and presence (green) of 50 nM μ-Conotoxin SxIIIC (taken from the recording in A).
Alomone Labs μ-Conotoxin SxIIIC inhibits Na_V1.7 channel currents heterologously expressed in Xenopus oocytes.
A. Representative time course of μ-Conotoxin SxIIIC (#STC-130) inhibition of Na_V1.7 channels current. Membrane potential was held at -100 mV, current was elicited by a 100 ms voltage step to 0 mV every 10 sec, and significantly inhibited by application of 1 µM μ-Conotoxin SxIIIC (green).
B. Superimposed traces of Na_V1.7 channel currents in the absence (control) and presence (green) of 1 µM μ-Conotoxin SxIIIC (taken from the recording in A).

References - Scientific background

McMahon, K.L. et al. (2020) Biomedicines, 8, 321.
Huang, W. et al. (2017) Protein Cell, 8, 401.
Mackieh, R. et al. (2021) Mar. Drugs, 19, 562.

Scientific background

µ-conotoxin SxIIIC (SxIIIC) is a 22 amino acid peptidyl toxin originally isolated from the venom of the cone snail, Conus striolatus¹. SxIIIC is a potent and irreversible blocker of voltage-gated sodium (Na_v) channels, which displays a unique µ-conotoxin selectivity profile of human (h)Na_V1.4 > hNa_V1.3 > hNa_V1.1 ≈ hNa_V1.6 ≈ hNa_V1.7 > hNa_V1.2 >> hNa_V1.5 ≈ hNa_V1.8¹.

Voltage-gated sodium channels (VGSCs) are transmembrane proteins that control the voltage-dependent increase in sodium permeability. VGSCs play a fundamental role in normal neurological function, especially in the initiation and propagation of action potentials. Na_v channels have been the topic of significant research and discussion for a considerable amount of time given their unique functions in electrical cell signaling. These channels are very important for homeostasis, thus specific genetic abnormalities in VGSC genes can result in a range of muscle, cardiac, and neurological disorders known as “channelopathies”². Marine toxins appear to be an emerging source of therapeutic tools that can relieve pain or treat VGSC-related human channelopathies³.

Target Nav1.4, Nav1.3, Nav1.1, Nav1.6 and Nav1.7 blocker

Peptide Content: 100%

Lyophilized Powder

Mu-conotoxin SxIIIC (#STC-130) is a highly pure, synthetic, and biologically active peptide toxin.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 07/05/2024

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µ-Conotoxin SxIIIC

Overview

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Scientific Background

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