…(and used under license from ATTO-TEC). ATTO-488. Maximum absorption 501 nm; maximum fluorescence 523 nm. The fluorescence is excited most efficiently in the 480 – 515 nm range. This…

Multiplex immunostaining:
Applications of ATTO primary conjugates

…photo-stability For more information on ATTO dye applications: Conjugated antibody guide for multiplex immunostaining Download New Technical Paper We have ~100 ATTO-conjugated primary antibodies, which we have used in combination…

Protected: Immunoassay Controls

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Multiplex Staining Updates

Alomone Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization in complex immunostaining or immunoprecipitation protocols: ATTO-Conjugated Antibodies These antibodies can be used in immunohistochemistry (IHC)…

Visualize the Color of Your Success
with ATTOs

…Alexa-488 ATTO-550 Orange λex 554 nm; λex 576 nm in 0.1 M phosphate pH 7.0 TAMRA, Cy3, Alexa-555 ATTO-594 Red λex 601 nm; λex 627 nm in 0.1 M phosphate…

Imaging ion channels with fluorescently labeled toxins

…of the two images demonstrates co-staining of GIRK2 channels by Tertiapin-Q-ATTO Fluor-488 and by Guinea pig Anti-GIRK2 (K_ir3.2) Antibody. DAPI staining (blue) is used to stain nuclei. Tityustoxin-Kα-ATTO Fluor-594 labels…

Putting Pressure on the Angiotensin Receptors

GPCR Pathways - Issue No. 4

…studying the subcellular role and distribution of Angiotensin receptors¹⁹. ATTO dye-labeled antibodies are also available; Anti-Angiotensin II Receptor Type-1 (extracellular)-ATTO Fluor-550 (#AAR-011-AO) and Anti-Angiotensin II Receptor Type-2 (extracellular)-ATTO Fluor-488 (#AAR-012-AG)…

Voltage-Gated Calcium Channel Modulators

Nicotinic Acetylcholine Receptor (nAChR) Agonists and Antagonists

Pharmacological Reagents

…GIRK2 channels in substantia nigra pars compacta using Tertiapin-Q-ATTO Fluor-488 and Guinea pig Anti-GIRK2 (K_ir3.2) Antibody. Tertiapin-Q-ATTO Fluor-488 (#STT-170-AG) Drug discovery Research interest in ion channels has without…

Voltage-Gated Sodium Channel Modulators

Ion Channels and Pain

Modulator - Issue No. 26

…DRG Immunohistochemical of TRPV1 and Na_V1.8 in rat dorsal root ganglion using Anti-Rat TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG), (green), (1:60) and Anti-Na_V1.8 (SCN10A)-ATTO Fluor-594 Antibody (#ASC-016-AR), (red), (1:60). A….

Live Cell Imaging Catalog

…detect specific cell types in heterogenous cell populations. They are available in unconjugated and conjugated formats. Conjugate labels include ATTO dyes, Phycoerythrin (PE) and Fluorescein isothiocyanate (FITC). We also are…

Flow Cytometry Frontiers: Alomone Labs’ Antibodies in Action

…the analysis of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients, with and without dexamethasone treatment. Flow cytometry, using our Mouse Anti-Human Orai1 (extracellular)-ATTO Fluor-633 Antibody (#ALM-025-FR) revealed that dexamethasone…

Antibody FAQs

…antibody. What are ATTO Fluor-conjugated antibodies? ATTO Fluor-conjugated antibodies are primary antibodies that contain an ATTO Fluor dye (fluorophore) directly attached to them, which eliminates the need for a secondary…

Custom Service

…Panel Development Product Information Catalog number Reporter type ATTO Fluor-488 ATTO Fluor-550 ATTO Fluor-590 ATTO Fluor-594 ATTO Fluor-633 ATTO Fluor-647N Pacific Blue Fluorescein isothiocyanate (FITC) R-Phycoerythrin (R-PE) Allophycocyanin (APC) Other…

Labeled Toxins for Ion Channels

…Tertiapin-Q-ATTO Fluor-488 and Guinea pig Anti-K_ir3.2 (GIRK2) Antibody. Tertiapin-Q-ATTO Fluor-488 (#STT-170-AG) Alomone Labs Tertiapin-Q-ATTO Fluor-633 binds GIRK1/GIRK4-GFP transfected HEK293T cells. Tertiapin-Q-ATTO Fluor-633 (#STT-170-FR) The pictures are a…

Synaptic markers for pre- and postsynaptic regions

…Antibody (#AGT-005) Anti-Vesicular GABA Transporter (VGAT)-ATTO Fluor-594 Antibody (#AGT-005-AR) Guinea pig Anti-Vesicular GABA Transporter (VGAT) Antibody (#AGP-129) Figure 11. Colocalization of GluR1 and Vesicular GABA Transporter in human U-87 MG…

NMDA Glutamate Receptors Antibodies, Agonists, and Antagonists

KCa Channels

K_Ca Channels Calcium-Activated Potassium Channel Modulators and Antibodies View our K_Ca Channel Products Calcium-activated potassium channels, also known as calcium-dependent potassium channels, play a crucial role in cellular signaling…

Antibodies, Small Molecules &
Peptide Toxins

…the extra mile ahead of everyone’s. Antibodies for Multiplex Staining Antibodies for Live Cell Imaging Antibodies for Live Cell Flow Cytometry ATTO/Biotin-Labeled Peptide Toxins Learn more about us……

Selecting the right antibody for the right job

…primary antibody conjugated directly to a reporter, like an ATTO fluorophore for example. Since you don’t need a secondary antibody for the direct method, it’s quicker, cheaper, and can reduce…

Venom-Derived Ion Channel Modulators

…Custom Toxin Development and Conjugation Services To allow visualization and functional studies, we offer toxins conjugated to reporters such as ATTO fluorophores, fluorescein isothiocyanate (FITC), or biotin. All of our…

Antibodies

…Labs has developed two innovative platforms for studying protein-protein interaction and/or protein co-localization: ATTO-conjugated antibodies Primary antibodies conjugated to ATTO fluorescent dyes: These antibodies are developed for immunohistochemistry (IHC) and…

In Focus: Formyl Peptide Receptors

GPCR Pathways - Issue No. 5

…(extracellular)-ATTO Fluor-488 antibody (#AFR-001-AG) and Anti-Human FPR2/ALX (extracellular) antibody (#AFR-002). All three antibodies are directed against extracellular epitopes and have been validated in indirect/direct flow cytometry. We are also pleased…

Live Cell Imaging Updates

Extracellular antibodies are ideal research tools for cell-surface proteins. No permeabilization/fixation needed, because using extracellular antibodies allows you to work with live cells! Benefits of live cell imaging: ✓ Monitor…

To Fight or to Flee from Adrenoceptors

GPCR Pathways - Issue No. 4

…Antibody (#AAR-015), Anti-α_1B-Adrenergic Receptor (extracellular) Antibody (#AAR-018), the labeled version, Anti-α_1B-Adrenergic Receptor (extracellular)-ATTO Fluor-488 Antibody (#AAR-018-AG), Anti-α_1D-Adrenergic Receptor (extracellular) Antibody (#AAR-019), Anti-α_2B-Adrenergic Receptor (extracellular) Antibody (#AAR-021), Anti-α_2C-Adrenergic Receptor (extracellular) Antibody…

Of Mitochondria and Stiff Hearts

…Rabbit | Applications: IFC, IHC, LCI, WB, *ICC, *IP | Reactivity: H, M, R Anti-Ca_V1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG) A fluorescent labeled primary antibody. It can be used in multiplex staining studies in…

Narirutin: Satsuma’s Chemical for Banishing Pain

…(#ASC-008-GP) Anti-Na_V1.7 (SCN9A)-ATTO Fluor-633 Antibody (#ASC-008-FR) If you need something more bespoke, like a custom conjugation, novel toxin, or just a bulk order, you can always use our Custom Services. …

TRPV1 Antibodies, Agonists, and Antagonists

When Pain Aids Cancer

…the same epitope as that of #ACC-030. Anti-Rat-TRPV1 (VR1) (extracellular)-ATTO Fluor-488 Antibody (#ACC-029-AG). A fluorescent labeled primary antibody. It can be used in multiplex staining studies in conjunction with any…

Understanding the Role of GluN3A in Brain Development

…(GluN2A) (extracellular) Antibody (#AGC-002) Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody (#AGC-002-AG) Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) Anti-PSD-95 Antibody (#APZ-009) Pharmacological Tools Blockers/Antagonists: small molecules MPX-004 (#M-280)…

Immunocytochemistry (ICC) Protocols for Fixed or Live Cells: Indirect and Direct Methods

…Assay Buffer and drain well. Add ICC-PBS to cover the cells and proceed with microscopy. OPTION B: Direct Labeling of Fixed Cells Using ATTO-Conjugated Primary Antibodies Add the ATTO-conjugated primary…

Immunohistochemistry (IHC) Protocols for Frozen Sections: Direct Methods

…ATTO fluorophore. Since the direct IHC method doesn’t require a secondary antibody, it is quicker, cheaper, and may reduce non-specific binding when compared to an indirect IHC protocol. However, you…

Immunohistochemistry (IHC) Protocols for Frozen Sections: Multiplex Staining

…antibodies. The sections were incubated with a cocktail of rabbit Anti-Calnexin conjugated with ATTO red (ACS-009-AR) and rabbit Anti-Presenilin conjugated with ATTO green (AIP-011-AG) antibodies. A) Calnexin staining (red) appeared…

Product Codes and Guinea Pig Antibodies

…AG, which means conjugated to ATTO-Fluor Green (488) Other version codes include GP = Guinea Pig AG = ATTO-Fluor green (488) AR = ATTO-Fluor red (594-590) FR = ATTO-Fluor far…

Microglia Markers

Venom Peptides and their Mimetics as Potential Drugs

Modulator - Issue No. 19

…possible effects on K_V1.1 and K_V1.2 channels in the brain. Stichodactyla toxin (ShK) (#STS-400, #STS-390 [Dalazatide, ShK-186 [SL5]], #STS-400-AR [ATTO-590], #STS-400-B [Biotinylated]), a toxin isolated form the venom of sea…

Immunohistochemistry (IHC) Protocols for Paraffin-embedded Sections

…a fluorophore, such as an ATTO-fluor. If you have any problems, please see our extensive troubleshooting guides. De-paraffinization and clear with alcohol Immerse your slides in the following solutions –…

Antibody Isotype Controls: What they are and why you need them

…have a few more isotype control antibodies to choose from, such as Unconjugated Carrier-free (no BSA or NaN₃) Conjugated to other fluorophores, like an ATTO-Fluor What is an Isotype Control?…

Switching off to switch pacemaker cells on

… HCN2 Blocking Peptide (#BLP-PC030) Anti-HCN2-ATTO Fluor-594 Antibody (#APC-030-AR) – A fluorescent-labeled primary antibody. It can be used in multiplex staining studies in conjunction with any of our antibodies raised…

TRPC1 and TRPC6: Driving Endotoxemic Cardiac Dysfunction

…Antibodies Anti-TRPC1 Antibody (#ACC-010) Anti-TRPC6 Antibody (#ACC-017) Anti-TRPC6 (extracellular) Antibody (#ACC-120) Anti-TRPC2 Antibody (#ACC-027) Anti-TRPC3 Antibody (#ACC-016) Anti-TRPC3-ATTO Fluor-594 Antibody (#ACC-016-AR) Anti-TRPC4 Antibody (#ACC-018) Anti-TRPC5 Antibody (#ACC-020) Anti-TRPC7 (extracellular) Antibody…

Acid-Sensing Ion Channels (ASICs)

How Rabies Modulates Macrophages

…on human MDMs (Figure 1). To test if RABV-G actually binds to this receptor, researchers used alpha-bungarotoxin conjugated directly to the fluorophore ATTO-633 (α-BTX; Alomone Labs, #B-100-FR), a potent antagonist…

Extracellular Conjugated Antibodies

…a straightforward path to achieving high-quality data. Flexible Fluorophores Our range of fluorophore-conjugated extracellular antibodies includes a diverse selection of fluorophore reporters such as ATTO Fluor across the spectrum from…

In Focus: Labeled α-Bungarotoxin

Modulator - Issue No. 26

…α-Bungarotoxin-ATTO Fluor-488 (#B-100-AG), labeled with a green fluorescent dye and α-Bungarotoxin-Biotin (#B-100-B), labeled with biotin. Alomone Labs α-Bungarotoxin-ATTO Fluor-488 in whole mount staining of mice Gastrocnemius muscle Whole mount staining…

Astrocytes in Epilepsy: Friend or Foe?

…(300-314)-ATTO Fluor-594 Antibody (#AQP-014-AR) Guinea Pig Anti-Aquaporin 4 (300-314) Antibody (#AQP-014-GP) Anti-Connexin-43 Antibody (#ACC-201) Anti-Connexin-37 Antibody (#ACC-204) Anti-Calnexin Antibody (#ACS-009) We also have blocking peptides to be used as controls…

Making Better Cardiac Pacemaker Cells

…Anti-HCN1 Antibody (#APC-056) Anti-HCN4 Antibody (#APC-052) Anti-NCX1 (SLC8A1) Antibody (#ANX-011) Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005) Anti-Connexin-40 (GJA5) Antibody (#ACC-205) Anti-Connexin-43 Antibody (#ACC-201) Anti-Connexin-45 (GJC1) Antibody (#ACC-207) Anti-CaV1.2 (CACNA1C) Antibody (#ACC-003) Anti-CaV1.2 (CACNA1C)-ATTO Fluor-488 Antibody (#ACC-003-AG) Anti-NaV1.5 (SCN5A) (493-511) Antibody (#ASC-005)…

Local protein synthesis changes cause neurodegeneration in ALS

…they do so by transmitting miRNA and mRNA loaded exosomes trans-synaptically.” Alomone reagents used in the research Postsynaptic marker For fluorescent stainings, the team used the labeled toxin, α-Bungarotoxin-ATTO Fluor-633…

Custom Services: Your Research Your Way

…choose from fluorescent tags like ATTO-Fluors (488, 590, 594, or 633) and FITC or even non-fluorescent tags like biotin. If you need something else, then we can almost certainly make…

Making Sure Microglia Are Microglia

…(#APR-020) Anti-P2Y12 Receptor Antibody (#APR-012) Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP) Anti-P2Y12 Receptor-ATTO Fluor-594 Antibody (#APR-012-AR) Anti-P2Y12 Receptor (extracellular)-PE Antibody (#APR-020-PE) Anti-P2Y12 Receptor (extracellular)-FITC Antibody (#APR-020-F) Anti-TMEM119 (extracellular) Antibody (#ANR-175) Anti-TMEM119 (extracellular)-FITC Antibody (#ANR-175-F)…

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