Name: Anti-KV4.2 Antibody
Brand: Alomone Labs
SKU: APC-023

Overview

Cat #: APC-023

Alternative Name Voltage-gated potassium channel subfamily D member 2, KCND2, Shal1, RK5

Lyophilized Powder yes

Type: Polyclonal

Host: Rabbit

Reactivity: h, m, r

Immunogen

Peptide (C)SNQLQSSEDEPAFVSK, corresponding to amino acid residues 454-469 of rat K_V4.2 (Accession Q63881). Intracellular, C-terminus.

Accession (Uniprot) Number Q63881

Gene ID 65180

Peptide confirmation Confirmed by amino acid analysis and mass spectrometry.

Homology Mouse, human - 15/16 amino acid residues identical.

RRID AB_2040176.

Purity Affinity purified on immobilized antigen.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.

Form Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Isotype Rabbit IgG.

Storage before reconstitution The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Reconstitution 25 µl, 50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.

Reconstitution 0.2 ml double distilled water (DDW).

Antibody concentration after reconstitution 0.6 mg/ml.

Storage after reconstitution The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Standard quality control of each lot Western blot analysis.

Applications: ic, if, ih, ip, wb

May also work in: ifc*

Western blot

Western blot analysis of rat brain membranes:
1. Anti-K_V4.2 Antibody (#APC-023), (1:200).
2. Anti-K_V4.2 Antibody, preincubated with Kv4.2 Blocking Peptide (#BLP-PC023).
Human myometrium tissue lysate (Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.).

Immunoprecipitation

Rat brain lysate (Chu, P.J. et al. (2006) J. Biol. Chem. 281, 365.).

Immunohistochemistry

Rat brain sections.

Human myometrium sections (1:50) (Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.).

Immunocytochemistry

Mouse spiral ganglion (Adamson, C.L. et al. (2002) J. Neuroscience 22, 1385.).

References

Roberds, S.L. and Tamkun, M.M. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 1798.
Serodio, P. et al. (1994) J. Neurophysiol. 72,1516.
Guo, W. et al. (2002) Circ. Res. 90, 586.
Escoubas, P. et al. (2002) Mol. Pharmacol. 62, 48.

Scientific background

K_V4.2 is a voltage-dependent K⁺ channel that belongs to the Shal channel subfamily and includes two other members: K_V4.1 and K_V4.3.¹

K_V4.2 possesses the signature structure of the voltage-dependent K⁺ channels: six membrane-spanning domains with intracellular N and C termini. As with other members of the voltage-gated K⁺ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of K_V4.1 and K_V4.3.

The K_V4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K⁺ channels. This type of current is known as transient A-type K⁺ currents. For example, depolarization-activated K⁺ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of K_V4.2 and K_V4.1 subunits.²

The biophysical properties of the K_V4.2 subunit can be modified by its association with auxiliary β subunits such as the KChIP family that increase K_V4.2 current densities and accelerates both the inactivation and the recovery time.

K_V4.2 is also highly expressed in the heart where together with K_V4.3 underlie the fast inactivating and recovering cardiac transient outward current I_to.³

Several toxins from spider venoms are potent blockers (affecting the channels in the nanomolar range) of K_V4.2 channels. Among these the most potent and selective are Stromatoxin-1 (#STS-350), (1.2nM), Phrixotoxin-1 (#STP-700), (5 nM), Phrixotoxin-2 (#STP-710), (34 nM) and Heteropodatoxin-2 (#STH-340), (100 nM).⁴

Application key:

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Species reactivity key:

H- Human, M- Mouse, R- Rat

Lyophilized Powder

Anti-K_V4.2 Antibody (#APC-023) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize K_V4.2 from human, rat, and mouse samples.

Certificate of Analysis SDS

For research purposes only, not for human use

Last Update: 29/10/2024

Applications

Citations

Western blot citations

Rat heart lysate.
Liu, X. et al. (2016) Cell. Physiol. Biochem. 39, 102.
Mouse heart lysate (1:2000).
El Gebeily, G. et al. (2015) J. Mol. Cell. Cardiol. 86, 85.
Rat ventricular myocytes (1:200).
Liu, W.J. et al. (2015) Am. J. Physiol. 309, H1288.
Mouse brain lysate (1:2000).
Hall, A.M. et al. (2015) J. Neurosci. 35, 6221.
Human myometrium tissue lysate.
Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.
Mouse sarcolemmal protein lysate.
Ednie, A.R. and Bennett, E.S. (2015) J. Biol. Chem. 290, 2769.
Rat uterine tissue lysate.
Novakovic, R. et al. (2013) J. Physiol. Pharmacol. 64, 795.

Immunoprecipitation citations

Rat brain lysate (2 µg).
Jensen, C.S. et al. (2014) J. Biol. Chem. 289, 10566.
Rat brain lysate.
Chu, P.J. et al. (2006) J. Biol. Chem. 281, 365.

Immunohistochemistry citations

Rat lumbar spinal cord sections.
Wolff, M. et al. (2016) Neurosci. Res. 109, 16.
Human myometrium sections (1:50).
Novakovic, R. et al. (2015) Mol. Hum. Reprod. 21, 545.

Immunocytochemistry citations

Mouse spiral ganglion.
Adamson, C.L. et al. (2002) J. Neuroscience 22, 1385.

More product citations

Kunert Keil, C. et al. (2013) J. Renin-Angiotensin-Aldosterone System 14, 41.
Kerti, K. et al. (2012) Eur. J. Neurosci. 35, 66.
Suzuki, T. et al. (2012) PLoS One 7, e35353.
Bignolais, O. et al. (2011) J. Mol. Cell. Cardiol. 51, 713.
Chen, M. et al. (2010) Am. J. Physiol. 299, R177.
Dabrowska, J. et al. (2010) Neuroscience 171, 721.
Liu, W. et al. (2010) J. Mol. Cell. Cardiol. 49, 438.
Moise, L. et al. (2010) Channels 4, 115.
Grandy, S.A. et al. (2009) J. Mol. Cell. Cardiol. 47, 238.
Loewen, M.L. et al. (2009) Am. J. Physiol. 296, H71.
Fernandez-Velasco, M. et al. (2007) Am. J. Physiol. Heart Circ. Physiol. 293, H238.
Sonner, P.M. and Stern, J.E. (2007) J. Physiol. 582, 1219.
Xia, F. et al. (2007) Endocrinology 148, 2157.
Colinas, O. et al. (2006) Am. J. Physiol. Heart Circ. Physiol. 291, H1978.
Hu, H-J. et al. (2006) Neuron 50, 89.
Jia, Y. et al. (2006) Circ. Res. 98, 386.
Petkova-Kirova, P.S. et al. (2006) Am. J. Physiol. Heart Circ. Physiol. 290, H2098.
Suzuki, T. et al. (2005) Am J. Physiol. Endocrinol. Metab. 288, E335.
Xu, Y. et al. (2005) J. Physiol. 562.3, 745.
Li, G.R. et al. (2003) Cardiovasc. Res. 58, 89.
Shimoni, Y. et al. (2003) Am. J. Physiol. Heart Circ. Physiol. 284, H1168.
Shimoni, Y. et al. (2003) J. Physiol. 550.2, 401.
Yamashita, T. et al. (2003) Circulation 107, 1917.
Amberg, G.C. et al. (2002) J. Physiol. 544.2, 403.
Amberg, G.C. et al. (2002) J. Physiol. 544.2, 417.
Sanchez, D. et al. (2002) J. Physiology 542.2, 369.
Trepanier-Boulay, V. et al. (2001) Circ. Res. 89, 437.
Adams, J.P. et al. (2000) J. Neurochem. 75, 2277.
Anderson, A.E. et al. (2000) J. Biol. Chem. 275, 5337.
Yamashita, T. et al. (2000) Circulation 101, 2007.

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Order Summary

Anti-K_V4.2 Antibody

Overview

Application key:

Species reactivity key:

Applications

Citations

Specifications

Scientific Background

Resources

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